Description:
Basedonourinnovativeandproprietarylipid-conjugationtechnology,LipoJet™TransfectionKit,formulatedfromnovelfluorinatedcationiclipids,exhibitssignificantdifferencefromotherlipidstransfectionreagentsinthemarket.LipoJet™TransfectionKitisthemostpowerfulyetverygentlegenedeliverytoolforavarietyofapplicationsincludingplasmidDNAand/or siRNAformostofmammaliancelltypes. ComparedwithleADIngproductsinthemarket, LipoJet™ismore cost-effectiveandalways provideshighertransfectionefficiencywithlesscytotoxicity.

Kitcontent:
-LipoJet™Reagent,1.0mlsufficientfor1000DNAtransfectionsand1000siRNAtransfections (24-wellplate).
-LipoJet™TransfectionBuffer(5x),8.0mLtomake40mLofworkingsolution(1x).

Features: 
-Versatile:DNAtransfection,siRNAtransfectionandDNA/siRNAco-transfection.
-Powerful: Outstandingefficiencyonadherentcells.
-Extremelylowtoxicity: ImproveCellviABIlityaftertransfection
-Economical:Moretransfectionswithlessreagent
-Easytouse:OnetubereactionandcompatIBLewithserum/antibiotics.
-Suitableforhigh-throughputapplication

Storage:
Storeat4°CforLipoJet™ReagentandRTforLipoJet™TransfectionBuffer(5x). Ifstoredproperly,theproductisstablefor12monthsorlonger.

BroadTransfectionSpectrumforMammalianCellTypes

ExamplesShowingExcellentDNAandsiRNATransfectionEfficiencyofLipoJet™InVitroTransfectionKit

AefficiencycomparisonofLipoJet™reagentvs.brandnameproductstotransfectHela,Cos-7,NIH-3T3,CHOandHEK293(leftpanel)andCos-7(rightpanel). pEGFP-N3CDNA(0.5µg/wellof24-wellplate)wastransfectedintodifferentmammaliancellsperthestandardtransfectionprotocolsinpresenceofserum(10%FBS)andantibioticsasrecommendedbymanufacturers. ThetransfectionefficiencywereanalyzedviaFACS48hoursposttransfection.


AtoxicitycomparisonofLipoJet™reagentvs.brandnameproductstotransfectHelacells. pEGFP-N3cDNA(1.0µg/wellof24-wellplate)wastransfectedtoHelacellsperthestandardtransfectionprotocolsinpresenceofserum(10%FBS)andantibioticsasrecommendedbymanufacturers. TheMTTassay(rightpanel)andphasecontrastimaging(leftpanel)wereusedtoanalyzethecellviability48hoursposttransfection.


AcomparisonofLipoJet™reagentvs.Lipofectamine2000(L2K)andPolyJet™transfectionreagentonHEK293Tcell.pEGFP-N3cDNA(0.125µg/well,0.25µg/welland0.5µg/perwellof24-wellplate)wastransfectedinto293Tcellsusingthestandardtransfectionprotocolsinpresenceofserum(10%FBS)withLipoJet™(upperpanelatLipoJet™/DNA(µl/µg)ratio=2),L2K(middlepanelatL2K/DNA(µl/µg)ratio=3)andPolyJet™(lowerpanelatatPolyJet™/DNA(µl/µg)ratio=3)respectively.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscope48hoursposttransfection.


AcomparisonofLipoJet™reagentvs.Lipofectamine2000(L2K)andPolyJet™transfectionreagentonHelacell. pEGFP-N3cDNA(0.125µg/well,0.25µg/welland0.5µg/perwellof24-wellplate)wastransfectedintoHelacellsusingthestandardtransfectionprotocolsinpresenceofserum(10%FBS)withLipoJet™(upperpanelatLipoJet™/DNA(µl/µg)ratio=2),L2K(middlepanelatL2K/DNA(µl/µg)ratio=3)andPolyJet™(lowerpanelatatPolyJet™/DNA(µl/µg)ratio=3)respectively.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscope48hoursposttransfection.




ExceptionalgenesilencingofLipoJet™reagentduringDNA/siRNAco-transfectiononmammaliancells.TransfectionofGFPcDNA(leftpanel,0.25µgperwellof24-wellplate)andco-transfectionofGFPcDNA(0.25µgperwellof24-wellplate)andGFPtargetedsiRNA(final10nM,rightpanel)withLipoJet™reagentleadstoexceptionalgenesilencingonHEK293(upperpanel),Hela(middlepanel)andSaoS-2(lowerpanel)cellsrespectively.ThecellswerevisualizedbyNikonEclipseFluorescencemicroscope48hoursposttransfection.


ExceptionalDNA/siRNAco-transfectionefficiencyonHUVEC.Co-transfectionofmCherrycDNA(0.10µgperwellof24-wellplate)andFITCconjugatedsiRNA(final30nMperwellof24-wellplate)toHUVECwithLipoJet™reagentgaveriseto60%mCherry+(phasecontrastoverlappedwithmCherryimaging,leftpanel)andnearly100%FITC-siRNA+(phasecontrastoverlappedwithFITCimaging,rightpanel)HUVEC24hoursaftertransfection. ThepicturesweregivenfromDr.PanKongofUSCascourtesy. 



ExcellentsilencingofendogenouslyexpressedKIF11(alsoknownasEG5)inHEK293(upperpanel)andHela(lowerpanel)cellswithLipoJet™reagentat10nMEG5siRNA.KIF11(alsoknownasEG5)encodesamotorproteinthatbelongstothekinesin-likeproteinfamilyinvolvedinchromosomepositioningandbipolarspindleformationduringcellmitosis.AreductioninKIF11levelscausesmitoticarrest.LipoJet™reagenteffectivelydeliversEG5siRNA(final10nM)toHEK293andHelacells,leadingtomorethan80%of"round-up"phenotypeofHEK293andHelacells24hposttransfectionovernegativecontrol(final10nMwithshamEG5siRNA).Thephenotypeof"rounded-up"HEK293andHelacellswerevisualized24hposttransfectionwithaNikonmicroscope.


AimageshowingexceptionaltransfectionefficiencyofLipoJet™TransfectionKitonHumanembryonicstemcells (hESCs). ThehESCsgrowninE8mediumonGeltrexvs(leftpanel,DICimaging)wastransfectedwithpEF1α-GFP.TheGFPexpression(rightpanel)wasvisualizedbyNikonEclipseFluorescencemicroscope24hoursposttransfection.TheabovepictureswereprovidedbyDr.MarinaPryzhkovaofJohnsHopkinsUniversityascourtesy

DataSheet&Protocol:
-AProtocolforDNAandsiRNATransfection
-AShortProtocolforDNATransfection 
-AShortProtocolforsiRNATransfection 
-AProtocolforDNA/siRNACo-transfection 
- TechnicalNote&TransfectionTips  

Weareoffering"WirelessPowerPointPresenter"tocustomerswhopurchase5x1.0mLLipoJet™reagentbyDecember2014.Takeadvantageofitanddon"tletthischancego...

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Testimonials:

Sorryforsooobigdelay,butnowIamtotallyinlovewithbothPolyJetandLipoJet.RecentlywediscoveredthatLipoJethassupergoodtransfectionefficiencywithmESCsandhESCswithi muchlesstoxicity.LastweekItransfectedovernighthumanESClineH1usingLipoJetwithsameamazinglyhighefficiency-upto50~70%.DNAconstructincludesGFPunderEF1apromoter(CMVpromoterdoesnotworkinhESCs).ThisisthebesttransfectionefficiencyIeversawwithESCs.Lipofectamin3000didn"tgivemetheseresults(usedonmouseESCs).

--------MarinaPryzhkova,Ph.D.,JHU

Itriedtheplasmid/siRNAco-transfectionusingtheLipoJetsampleinEAhy926cellwhichisaHUVECcellline.Theresultsareprettygood,atleastforthetransfectionefficiency.Fortheplasmidtransfection,48hourslater,around60%cellaretransduced.ForsiRNA,theefficiencyisalmost100%.WeareorderingmoreLipoJetreagenttousefromnow.

-----Dr.PanKong,UniversityofSouthernCalifornia

Wegot>90%efficiencywithLipoJetvs.80%withX-tremeGENE9on293Tcell.Definitelywillordermore....

-----Dr.NuoYangfromRoswellParkCancerInstitute

HerearetheresultsfromourpreliminaryexperimentswithLipoJet.Weareverysatisfiedwithitsefficiency.Unfortunately,wewereoutofFugene6/HDandwereunabletotestthemsidebyside,butbasedonpreviousexperience,wedobelievethatyourproductworkedwithbetterefficiency.

------Dr.AlisonMcKelveyfromUniversityofPittsburgh

IamhappytosaythatIhadgreatsuccesswithyourtransfectionreagentsonOKF6/TERT2humankeratinocytes.IwillputtogetherthedataonceIhavecompletedmyanalysis.IreallyliketheLipoJet.MycellofinterestdidnotdoverywellintheGenMutealthoughmycontrolcellsdid.WewanttogoaheadwithorderingASAP.Ialsohaveacouponfor10%foraPOorder.

-----Dr.MichelleSimpson-AbelsonfromUPMC

WedidindeedtestthemsidebysidewithourhomemadeCaPhos.Theresultsaregreat, LipoJetandCalFectinbothdidextremelywell.LipoJetbeingthebetterofthetwo.Iwillsendyoutheresultswhen Igetthem, Iamcurrentlyoutofthelabbutwewillbeorderingmoreforcertain.Thankyouforsendingthosesamples.

------Dr.AhmedHassib,CornellUniversity

FortheLipoJet,IonlycompareditwithLipoLTXinHelacells.TheLipoJetgaveamuchhigherefficiency(~35%betterthanLipoLTX)24hoursaftertransfection.WewillordersomeLipoJet.

-----AbetatesterfromUSC