ProductCategory:HumanAdenovirusType5(dE1/E3);Ad-CMV-GCaMP6s;Ad-GCaMP6s
Description:
GCaMPisageneticallyencodedcalciumindicator(GECI)andcreatedfromafusionofgreenfluorescentprotein(GFP),calmodulin(CaM),andM13,apeptidesequencefrommyosinlightchainkinase. WhenGECIbindsCa2+,theconformationalchangeofGCaMP inducesproximityofthe GFPparts,eliciting strongGFPfluorescencesignal,whichallowsmeasuringactionpotentialsandotherreceptoractivationeventsthattriggerCa2+influx.TheadvantageofGECI'sarethattheycanbegeneticallyspecifiedforstudiesinlivingorganisms.
GCaMP6isanovelultra-sensitiveformatofGCaMPthatoutperformedothersensorsintermsofaccuracyandsensitivityinmeasuringcytosolCa2+level. MutationsincalmodulinpartofGCaMP6createdseveralvariants whichshoweddifferentcalciumfluorescencedecayrate(fast- GCamP6f, slow -GCaMP6s andmedium-GCaMP6m).
Thisrecombinanthumanadenovirustype5expressesgeneticallyencodedcalciumindicatorGCaMP6s underCMVpromoter.
Titer:1x1010~1x1011PFU/mL
StorageBuffer:DMEMwith2%BSA&2.5%Glycerol
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