ProductCategory:humanAdenovirusType5(dE1/E3),mCherry-PA-Rac1adenovirusforover-expressionofmCherry-PA-Rac1underCMVpromoter;Ad-CMV-mCherry-PA-Rac1
Description:Rac1isakeyGTPaseregulatingactincytoskeletaldynamicsinmetazoancells.Rac1mutantswerefusedtothephotoreactiveLOV(lightoxygenvoltage)domainfromphototropin,stericallyblockingRac1interactionsuntilirrADIationunwoundahelixlinkingLOVtoRac1.PhotoactivatableRac1(PA-Rac1)couldbereversIBLyandrepeatedlyactivatedusing458-or473-nmlighttogeneratepreciselylocalizedcellprotrusionsandruffling.LocalizedRacactivationorinactivationwassufficienttoproducecellmotilityandcontrolthedirectionofcellmovement.MyosinwasinvolvedinRaccontrolofdirectionalitybutnotinRac-inducedprotrusion,whereasPAKwasrequiredforRac-inducedprotrusion.PA-Rac1wasusedtoelucidateRacregulationofRhoAincellmotility.RacandRhocoordinatecytoskeletalbehaviourswithsecondsandsubmicrometreprecision.AmCherryreporterwasthenclonedatN-terminalofPA-Rac1togeneratemCherry-PA-Rac1constructwhichwasthenpackagedtoadenovirus. Ad-CMV-mCherry-PA-Rac1canbeusedtoinfectmostofmammaliancelltypesfortime-lapsevisualizationofRac1activationviaphotoactivation.
Titer:1x1010~1x1011PFU/mL
StorageBuffer:DMEMwith2%BSA&2.5%Glycerol
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