>
产品中心 >
Cell_transfection >
Signagen/GenJet In Vitro DNA Transfection Reagent for Epithelial Cell/SL100489-EPI/0.5 mL
Description
GenJet DNA In Vitro
Transfection Reagent for
epithelial cell is pre-optimized for epithelial
cells. By cross-linking a peptide ligand, this pre-optimized
GenJet reagent specifically
binds epithelial cells
and give superior efficiency on epithelial cells.
Refer to the following optimal transfection conditions for maximal transfection
efficiency on epithelial cells. GenJet reagent, 1.0 ml, is sufficient for 600 to
1200 transfections in 24 well plates or 300 to 600 transfections in 6 well plates.
|
Summary of
Optimal Transfection Conditions: |
|
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A comparison showing superior efficiency of the
pre-optimized GenJet (Ver.
II) on normal human colonic epithelial
cells. Transfection was performed per the vendors' protocol. 0.5
µg
of GFP DNA (pEGFP-N3) per well in 24-well plate was delivered to normal human
colonic epithelial cells with different transfection methods. The GFP
positive cells were counted under a Nikon 2000 Eclipse fluorescence microscope
24 hours post transfection.
Data Sheet
