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Signagen/GenJet In Vitro DNA Transfection Reagent for L929 Cell/SL100489-L929/0.1 mL
Description
GenJet DNA In Vitro
Transfection Reagent for
L929 cell is pre-optimized for transfecting L929 cells.
L929 is a murine aneuploid fibrosarcoma cell
line which is often used to assay TNF-alpha and TNF-beta. Treatment with TNF
initiates apoptosis and subsequent cell death. The level of detection is
on the order of 50 pg/mL. Another cell line which is approximately 5-10-fold
more sensitive is WEHI-164. Some L929 subclones have been developed that allow
detection of TNF at concentrations of approximately 30 pmol/L (approximately 500
fg/mL). Sublines that are resistant to TNF have been established also.
Refer to the following optimal transfection conditions for maximal transfection
efficiency on L929 cells. GenJet reagent, 1.0 ml, is sufficient for 300 to
600 transfections in 24 well plates or 150 to 300 transfections in 6 well plates.
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Summary of
Optimal Transfection Conditions: |
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Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
Data Sheet
