>
产品中心 >
Cell_transfection >
Signagen/GenJet In Vitro DNA Transfection Reagent for MEFs/SL100489-MEF/5 x 1.0 mL
Description
GenJet DNA In Vitro
Transfection Reagent for MEFs is pre-optimized and conditioned for transfecting
Mouse Embryonic Fibroblasts (MEFs).
MEFs are required to support the growth of undifferentiated mouse or
human ES cells, iPS cells, thus they are also called feeder cells. MEFs
are isolated from mouse embryos and are used at their early passages.
Before MEFs are used as feeder cells,
they must be treated by
irradiation or mitomycin C to stop the cells from further dividing.
However MEFs are nortoriously hard to transfect.
With the pre-optimized reagent, you will get up to 85% GFP+ MEFs.
The "Shaved Cell
Transfection" protocol was optimized to achieve maximal transfection efficiency. Refer to the following optimal transfection conditions for maximal transfection
efficiency on MEFs cells. GenJet reagent, 1.0 ml, is sufficient for 500 transfections in 24 well plates or
250 transfections in 6 well plates.
|
Summary of
Optimal Shaved CellTransfection Conditions: |
|
Storage Condition
Store at 4 °C. If stored properly, the product is stable for 12 months or longer
A Picture Showing Transfection Efficiency of GenJet Reagent on MEFs. MEFs
were grown per ATCC recommended culture medium and transfected with pEGFP-N3 by
GenJet. The efficiency was checked 24 hours post transfection
Data Sheet
