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Signagen/GenMute siRNA Transfection Reagent for C2C12 Cell/SL100568-C2C12/1.0 mL
Description:
GenMute
siRNA Transfection Reagent for C2C12 Cell is pre-optimized for transfecting
siRNA to C2C12 cells. C2C12
cells were originally obtained by Yaffe and Saxel (1977) through selective serial passage
of myoblasts cultured from the thigh muscle of C3H mice 70 h after a crush
injury. These cells were shown to be capable of differentiation. C2C12 cells are
a useful model to study the differentiation of non-muscle cells to skeletal
muscle cells (e.g myosin phosphorylation mechanisms) and express muscle proteins
and the androhen receptor (AR).
Size & content:
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GenMute Reagent,
1.0 ml, sufficient for ~833 reactions based on transfecting 20 pmoles
siRNA or miRNA mimics in 24-well plate
-
GenMute Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml
to make 40 ml working solution
Refer to the following optimal transfection conditions for maximal silencing on C2C12 cells.
GenMute reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or
~416 transfections in 6 well plates.
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Summary of
Optimal Transfection Conditions: |
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Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or
longer.
Data Sheet & Protocol
