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Signagen/GenMute siRNA Transfection Reagent for Primary Neurons/SL100568-PNN/0.1 mL
Description:
GenMute
siRNA Transfection Reagent for Primary Neurons is pre-optimized for transfecting
siRNA to primary neurons. Primary neurons are notoriously heard to
transfect. With the pre-optimized
siRNA transfection conditions, GenMute reagent was
confirmed to give up to 90% silencing at final 50 nM siRNA on primary cultured
neurons.
Size & content:
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GenMute Reagent,
1.0 ml, sufficient for ~833 reactions based on transfecting 25 pmoles
siRNA or miRNA mimics in 24-well plate
-
GenMute Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml
to make 40 ml working solution
Refer to the following optimal transfection conditions for maximal silencing on
primary neurons. GenMute reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or
~416 transfections in 6 well plates.
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Summary of
Optimal Transfection Conditions: |
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Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or
longer.
Data Sheet & Protocol
