>
产品中心 >
Cell_transfection >
Signagen/GenMute siRNA Transfection Reagent for U2OS Cell/SL100568-U2OS/1.0 mL
Description:
GenMute
siRNA Transfection Reagent for U2OS Cell is pre-optimized for transfecting siRNA
to U2OS cells. The U2OS cell line, originally known as the 2T line, was cultivated from the
bone tissue of a fifteen-year-old human female suffering from osteosarcoma.
Established in 1964, the original cells were taken from a moderately
differentiated sarcoma of the tibia. U2OS cells exhibit epithelial
adherent morphology and viruses were not detected in the line during
co-cultivation with WI-38 cells or in CF tests against SV40, RSV, or
adenoviruses. Cells are positive for insulin-like growth factor I (IGF-I) and
insulin-like growth factor II (IGF II) receptors and express a number of
antigens, including blood type A, Rh+, HLA A2, Aw30, B12, Bw35, and B40(+/-).
Size & content:
-
GenMute Reagent,
1.0 ml, sufficient for ~833 reactions based on transfecting 15 pmoles
siRNA or miRNA mimics in 24-well plate
-
GenMute Transfection Buffer (5x ), formulated for maximal transfection efficiency, 8.0 ml
to make 40 ml working solution
Refer to the following optimal transfection conditions for maximal silencing on
U2OS cells.
GenMute reagent, 1.0 ml, is sufficient for ~833 transfections in 24 well plates or
~416 transfections in 6 well plates.
|
Summary of
Optimal Transfection Conditions: |
|
Storage:
Store at 4 °C. If stored properly, the product is stable for 12 months or
longer.
Data Sheet & Protocol
