ProductCategory:Pre-maderecombinantlentivirus;LV-EF1α-FLPo-Puro;LV-EF1A-FLPo-Puro;LV-EF1α-FLPo-IRES-Puro;EF1A-FLPo-PuroLentivirus;EF1α-FLPo-PuroLentivirus
Description: FLPoriginallyidentifiedfromSaccharomycescerevisiaeisasite-specificrecombinases(SSR)fromtheλintegrasefamilyandrecognizesdistinct34bpFRTsequence.Ithasbeenwidelyusedforgeneratingconditionalknockoutmice,mediatedbyFLP/FRTsystem.InitialuseofFLPinmammaliancellsrevealedinefficientrecombinaseactivityduetoThermo-instABIlityoftheFLPprotein.Subsequentscreeningforthermo-stablemutantsresultedintheidentificationofFLPewhichwas4-foldincreaseinrecombinationefficiencyat37°C.HowevertherecombinationefficiencyofFLPeincellsremainsverylowbecauseofitsnon-mammalianorigin.
FLPoisamousecodonoptimizedFLPethatgreatlyincreasestheFRTrecombinationefficiencyforbothinvivoandinvitroinmouse.ThisLV-EF1α-Cre-Puropre-made lentivirus expressesFLPorecombinasewithco-expressionofpuromycinselectioninanIREScassetteunderEF1αpromoter.Readytouseformat.
Titer:>1x109TU/mL
SelectionAntibiotic:Puromycin
Purity*: InvivogradeandOKforbothinvitrotissuecultureinfectionandinvivoinjection
Size: 2x25 µL(StdPack)
StorageBuffer:PBS
InfectionProtocol:
TransferVectorMap:
