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Signagen/shRNA/miRNA/gRNA Adenovirus Production Full Service Large Scale/SL100782/
ProductionofRecombinantSEROtype5shRNA/miRNA/gRNAAdenovirus(E1/E3deletion)withAd.MAXSystem:
-One-stopservice:fromshRNA/miRNAdesigntopackaging.
-Unsurpassabletransductionefficiency.
-Quickturnaroundtime:3to4weeks.
-Cost-effective.
-Specialdesignrequestacceptable,FREE!
LargeshRNA/miRNA/gRNAAd.MAX™shuttlevectorcollectionforcloningshuttleplasmid with aspecificpromoterandreporter:
Single-promoterAd.MAX™shuttlevector | Dual-promoterAd.MAX™shuttlevector | |||
Promoterless |
| Promoterless | CMV | eGFP |
ServiceDescription:
1.Design,synthesizeandcloneshRNA/miRNA/gRNAtoashuttlevectorfollowedbytransferofthewholetransgenecassettetoadenoviralgenome.
2.Initialadenoviralstockproduction.
3.Adenovirusamplificationfrom~4000cm2Ad.MAX™293cells.
4.Adenoviruspurificationvia2xCsClultracentrifugation.
5.RecombinantadenovirusPFUtitration.
RequiredMaterials:NCBIaccession#oftargetgene,targetregionsequence,shRNAorsiRNAsequencevalidated/publishedormaturemiRNAsequence,miRNAaccession#orgRNAsequenceetc.
TurnaroundTime:3~4weeks.
Deliverables:2mL(10x0.2mL)ofsuperpurifiedinvivogradeadenovirusvectorat5E+10~5E+11PFU/mL*.
Weofferdiscountfornewcustomer,pleaserequestaquotewithustoday.
WealsooffertruncatedshRNA/miRNA/gRNAservice,pleaserequestaquotewithustoday.
*Finaladenovirusyieldmaydependonthenatureoftransgene.