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Signagen/shRNA/miRNA/gRNA Adenovirus Production Full Service Pilot Scale/SL100785/
ProductionofRecombinantSEROtype5shRNA/miRNAAdenovirus(E1/E3deletion)withAd.MAXSystem:
-One-stopservice:fromshRNA/miRNAdesigntopackaging.
-Unsurpassabletransductionefficiency.
-Quickturnaroundtime:3to4weeks.
-Cost-effective.
-Specialdesignrequestacceptable,FREE!
LargeshRNA/miRNA/gRNAAd.MAX™shuttlevectorcollectionforcloningshuttleplasmid with aspecificpromoterandreporter:
Single-promoterAd.MAX™shuttlevector | Dual-promoterAd.MAX™shuttlevector | |||
Promoterless |
| Promoterless | CMV | eGFP |
ServiceDescription:
1.SynthesizeandcloneshRNA/miRNAtoashuttlevectorfollowedbytransferofthewholetransgenecassettetoadenoviralgenome.
2.ViralproductioninAd.MAX™293cells:transfectionandpurificationofrecombinantadenovirus.
3.Generationofhightiterrecombinantadenoviralstockin~500cm2Ad.MAX™293cells.
4.RecombinantadenovirusPFUtitration.
RequiredMaterials:NCBIaccession#oftargetgene,targetregionsequence,shRNAorsiRNAsequencevalidated/publishedormaturemiRNAsequence,miRNAaccession#orgRNAsequenceetc.
TurnaroundTime:3~4weeks.
Deliverables:1.0mL(5x0.2mL)ofsuperinfectiousinvitrogradeadenovirusvectorat5E+10~5E+11PFU/mL*.
Weofferdiscountfornewcustomer,pleaserequestaquotewithustoday.
WealsooffertruncatedcustomshRNA/miRNA/gRNAadenovirusservice,pleaserequestaquotewithustoday.
*Finalviralyieldmaydependonthetransgeneinthevirus.